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miscript ii reverse transcriptase kit  (Qiagen)


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    Structured Review

    Qiagen miscript ii reverse transcriptase kit
    Miscript Ii Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+kit+ii+miscript/Reverse+transcriptase/pm41611768-118-23-28
    Average 97 stars, based on 6786 article reviews
    miscript ii reverse transcriptase kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Circulatory extracellular vesicle derived miR-195-5p promotes cellular apoptosis and suppresses cell proliferation in the buffalo endometrial primary cell culture.
    Article Snippet: The RNA concentration for each sample was measured using NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μl concentration range was obtained from each sample. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat# 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. ..

    Article Title: Circulatory extracellular vesicle derived miR-195-5p promotes cellular apoptosis and suppresses cell proliferation in the buffalo endometrial primary cell culture
    Article Snippet: The RNA concentration for each sample was measured using NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μl concentration range was obtained from each sample. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat# 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. ..

    Article Title: Differential abundance of microRNAs in seminal plasma extracellular vesicles (EVs) in Sahiwal cattle bull related to male fertility
    Article Snippet: The miRNA was isolated from the sperm of HF (n = 3) and LF (n = 3) using the Qiagen Advanced miRNeasy Serum/plasma kit (cat. 217204, Qiagen, Germany) according to the manufacturer’s instructions, with the addition of bacteriophage MS2 RNA (10165948001, Roche) as a carrier RNA (to improve the RNA yield) in a 20 μL volume. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat# 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. miRNA, cel-miR-39-3p, was used as an exogenous spike-in control. .. The RNA concentration for each sample was measured using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μL concentration range was obtained from each sample.

    Article Title: Differential abundance of microRNAs in seminal plasma extracellular vesicles (EVs) in Sahiwal cattle bull related to male fertility
    Article Snippet: The RNA concentration for each sample was measured using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μL concentration range was obtained from each sample. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat # 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. ..

    Reverse Transcription:

    Article Title: Circulatory extracellular vesicle derived miR-195-5p promotes cellular apoptosis and suppresses cell proliferation in the buffalo endometrial primary cell culture.
    Article Snippet: The RNA concentration for each sample was measured using NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μl concentration range was obtained from each sample. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat# 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. ..

    Article Title: Low levels of exosomal-miRNAs in maternal blood are associated with early pregnancy loss in cloned cattle
    Article Snippet: .. Reverse transcription (RT) was performed using the Reverse Transcriptase Kit II miScript (Qiagen, Valencia, CA) according to the manufacturer’s recommendations. ..

    Article Title: Circulatory extracellular vesicle derived miR-195-5p promotes cellular apoptosis and suppresses cell proliferation in the buffalo endometrial primary cell culture
    Article Snippet: The RNA concentration for each sample was measured using NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μl concentration range was obtained from each sample. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat# 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. ..

    Article Title: Differential abundance of microRNAs in seminal plasma extracellular vesicles (EVs) in Sahiwal cattle bull related to male fertility
    Article Snippet: The miRNA was isolated from the sperm of HF (n = 3) and LF (n = 3) using the Qiagen Advanced miRNeasy Serum/plasma kit (cat. 217204, Qiagen, Germany) according to the manufacturer’s instructions, with the addition of bacteriophage MS2 RNA (10165948001, Roche) as a carrier RNA (to improve the RNA yield) in a 20 μL volume. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat# 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. miRNA, cel-miR-39-3p, was used as an exogenous spike-in control. .. The RNA concentration for each sample was measured using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μL concentration range was obtained from each sample.

    Article Title: Low levels of exosomal-miRNAs in maternal blood are associated with early pregnancy loss in cloned cattle.
    Article Snippet: .. Reverse transcription (RT) was performed using the Reverse Transcriptase Kit II miScript (Qiagen, Valencia, CA) according to the manufacturer’s recommendations. ..

    Article Title: Differential abundance of microRNAs in seminal plasma extracellular vesicles (EVs) in Sahiwal cattle bull related to male fertility
    Article Snippet: The RNA concentration for each sample was measured using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μL concentration range was obtained from each sample. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat # 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. ..

    Control:

    Article Title: Differential abundance of microRNAs in seminal plasma extracellular vesicles (EVs) in Sahiwal cattle bull related to male fertility
    Article Snippet: The miRNA was isolated from the sperm of HF (n = 3) and LF (n = 3) using the Qiagen Advanced miRNeasy Serum/plasma kit (cat. 217204, Qiagen, Germany) according to the manufacturer’s instructions, with the addition of bacteriophage MS2 RNA (10165948001, Roche) as a carrier RNA (to improve the RNA yield) in a 20 μL volume. .. Approximately 120 ng of the isolated RNA was converted into cDNA for each sample using the Reverse Transcriptase Kit II miScript (Cat# 218161 Qiagen, Valencia, CA) using HiSpec buffer (5X), miScript nucleic acid mix, and miScript enzyme (RT) in a final reaction volume of 20 μL for mature miRNA profiling. miRNA, cel-miR-39-3p, was used as an exogenous spike-in control. .. The RNA concentration for each sample was measured using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and a 10–15 ng/μL concentration range was obtained from each sample.



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